If you have the patient’s absolute lymphocyte count (ALC), call 513-636-4685 or use the “Customized Volumes by ALC”sheet to obtain the precise amount of blood required for this assay. If you do not have an ALC available, 5-10mL blood in Sodium Heparin (Dark Green) is the general requirement and should be sufficient for patients who are not lymphopenic.
Minimum Collection Volume
Minimum volume dependent on ALC
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Specimens collected in anticoagulants other than Sodium Heparin.
Storage/Transport Temperature
CRITICAL AMBIENT
Specimen Preparation
Transport whole blood in original collection tubes. Do not refrigerate or freeze. Specimen must be received by our laboratory within one day of collection. LIVE LYMPHOCYTES REQUIRED.
Cytotoxic T lymphocytes (CTL) are a subset of lymphocytes that have the ability to lyse target cells bearing specific antigens. To assess the functionality of CTLs, target cells previously primed to contain radioactive chromium within the cell membrane are incubated with patient or control lymphocytes. Lysis of the target cells by the CTLs is measured by the amount of chromium released into the supernatant.
CPT Codes
86352
Collection
Collect
If you have the patient’s absolute lymphocyte count (ALC), call 513-636-4685 or use the “Customized Volumes by ALC”sheet to obtain the precise amount of blood required for this assay. If you do not have an ALC available, 5-10mL blood in Sodium Heparin (Dark Green) is the general requirement and should be sufficient for patients who are not lymphopenic.
Minimum Collection Volume
Minimum volume dependent on ALC
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Specimens collected in anticoagulants other than Sodium Heparin.
Storage/Transport Temperature
CRITICAL AMBIENT
Specimen Preparation
Transport whole blood in original collection tubes. Do not refrigerate or freeze. Specimen must be received by our laboratory within one day of collection. LIVE LYMPHOCYTES REQUIRED.
Cytotoxic T lymphocytes (CTL) are a subset of lymphocytes that have the ability to lyse target cells bearing specific antigens. To assess the functionality of CTLs, target cells previously primed to contain radioactive chromium within the cell membrane are incubated with patient or control lymphocytes. Lysis of the target cells by the CTLs is measured by the amount of chromium released into the supernatant.
